Three-dimensional solution structure of beta cryptogein, a beta elicitin secreted by a phytopathogenic fungus Phytophthora cryptogea.
Identifieur interne : 002A35 ( Main/Exploration ); précédent : 002A34; suivant : 002A36Three-dimensional solution structure of beta cryptogein, a beta elicitin secreted by a phytopathogenic fungus Phytophthora cryptogea.
Auteurs : S. Fefeu [France] ; S. Bouaziz ; J C Huet ; J C Pernollet ; E. GuittetSource :
- Protein science : a publication of the Protein Society [ 0961-8368 ] ; 1997.
Descripteurs français
- KwdFr :
- Conformation des protéines (MeSH), Deutérium (MeSH), Données de séquences moléculaires (MeSH), Isotopes de l'azote (MeSH), Maladies des plantes (microbiologie), Modèles moléculaires (MeSH), Phytophthora (composition chimique), Phytophthora (pathogénicité), Protéines d'algue (MeSH), Protéines fongiques (composition chimique), Résonance magnétique nucléaire biomoléculaire (MeSH), Solutions (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Phytophthora, Protéines fongiques.
- microbiologie : Maladies des plantes.
- pathogénicité : Phytophthora.
- Conformation des protéines, Deutérium, Données de séquences moléculaires, Isotopes de l'azote, Modèles moléculaires, Protéines d'algue, Résonance magnétique nucléaire biomoléculaire, Solutions, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Algal Proteins (MeSH), Amino Acid Sequence (MeSH), Deuterium (MeSH), Fungal Proteins (chemistry), Models, Molecular (MeSH), Molecular Sequence Data (MeSH), Nitrogen Isotopes (MeSH), Nuclear Magnetic Resonance, Biomolecular (MeSH), Phytophthora (chemistry), Phytophthora (pathogenicity), Plant Diseases (microbiology), Protein Conformation (MeSH), Solutions (MeSH).
- MESH :
- chemical , chemistry : Fungal Proteins.
- chemical : Algal Proteins, Deuterium, Nitrogen Isotopes, Solutions.
- chemistry : Phytophthora.
- microbiology : Plant Diseases.
- pathogenicity : Phytophthora.
- Amino Acid Sequence, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Protein Conformation.
Abstract
Cryptogein belongs to a new family of 10-kDa proteins called elicitins. Elicitins are necrotic and signaling proteins secreted by Phytophthora spp. responsible for the incompatible reaction and systemic hypersensitive-like necroses of diverse plant species leading to resistance against fungal or bacterial plant pathogens. The solution structure of beta cryptogein from Phytophthora cryptogea fungus was determined by using multidimensional heteronuclear nuclear magnetic resonance spectroscopy. A set of 18 structures was calculated using 1360 NOE-derived distance restraints and 40 dihedral angle restraints obtained from 3JHNH alpha couplings. The RMS deviation from the mean structure is 0.87 +/- 0.14 A for backbone atoms and 1.34 +/- 0.14 A for all the non-hydrogen atoms of residues 2 to 98. The structure of beta cryptogein reveals a novel protein fold, with five helices and a double-stranded beta-sheet facing an omega-loop. One edge of the beta-sheet and the adjacent face of the omega-loop form a hydrophobic cavity. This cavity made of highly conserved residues represents a plausible binding site. Residue 13, which has been identified from directed mutagenesis and natural sequence comparison studies as a key amino acid involved in the differential control of necrosis, is surface exposed and could contribute to the binding to a ligand or a receptor. The solution structure is close to the X-ray structure, with slight differences lightly due to the crystal packing.
DOI: 10.1002/pro.5560061101
PubMed: 9385630
PubMed Central: PMC2143581
Affiliations:
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Guittet</name></author></analytic><series><title level="j">Protein science : a publication of the Protein Society</title><idno type="ISSN">0961-8368</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algal Proteins (MeSH)</term><term>Amino Acid Sequence (MeSH)</term><term>Deuterium (MeSH)</term><term>Fungal Proteins (chemistry)</term><term>Models, Molecular (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Nitrogen Isotopes (MeSH)</term><term>Nuclear Magnetic Resonance, Biomolecular (MeSH)</term><term>Phytophthora (chemistry)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>Protein Conformation (MeSH)</term><term>Solutions (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Conformation des protéines (MeSH)</term><term>Deutérium (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Isotopes de l'azote (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Modèles moléculaires (MeSH)</term><term>Phytophthora (composition chimique)</term><term>Phytophthora (pathogénicité)</term><term>Protéines d'algue (MeSH)</term><term>Protéines fongiques (composition chimique)</term><term>Résonance magnétique nucléaire biomoléculaire (MeSH)</term><term>Solutions (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Algal Proteins</term><term>Deuterium</term><term>Nitrogen Isotopes</term><term>Solutions</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Phytophthora</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Protein Conformation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term><term>Deutérium</term><term>Données de séquences moléculaires</term><term>Isotopes de l'azote</term><term>Modèles moléculaires</term><term>Protéines d'algue</term><term>Résonance magnétique nucléaire biomoléculaire</term><term>Solutions</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cryptogein belongs to a new family of 10-kDa proteins called elicitins. Elicitins are necrotic and signaling proteins secreted by Phytophthora spp. responsible for the incompatible reaction and systemic hypersensitive-like necroses of diverse plant species leading to resistance against fungal or bacterial plant pathogens. The solution structure of beta cryptogein from Phytophthora cryptogea fungus was determined by using multidimensional heteronuclear nuclear magnetic resonance spectroscopy. A set of 18 structures was calculated using 1360 NOE-derived distance restraints and 40 dihedral angle restraints obtained from 3JHNH alpha couplings. The RMS deviation from the mean structure is 0.87 +/- 0.14 A for backbone atoms and 1.34 +/- 0.14 A for all the non-hydrogen atoms of residues 2 to 98. The structure of beta cryptogein reveals a novel protein fold, with five helices and a double-stranded beta-sheet facing an omega-loop. One edge of the beta-sheet and the adjacent face of the omega-loop form a hydrophobic cavity. This cavity made of highly conserved residues represents a plausible binding site. Residue 13, which has been identified from directed mutagenesis and natural sequence comparison studies as a key amino acid involved in the differential control of necrosis, is surface exposed and could contribute to the binding to a ligand or a receptor. The solution structure is close to the X-ray structure, with slight differences lightly due to the crystal packing.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9385630</PMID><DateCompleted><Year>1998</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0961-8368</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><Issue>11</Issue><PubDate><Year>1997</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Protein science : a publication of the Protein Society</Title><ISOAbbreviation>Protein Sci</ISOAbbreviation></Journal><ArticleTitle>Three-dimensional solution structure of beta cryptogein, a beta elicitin secreted by a phytopathogenic fungus Phytophthora cryptogea.</ArticleTitle><Pagination><MedlinePgn>2279-84</MedlinePgn></Pagination><Abstract><AbstractText>Cryptogein belongs to a new family of 10-kDa proteins called elicitins. Elicitins are necrotic and signaling proteins secreted by Phytophthora spp. responsible for the incompatible reaction and systemic hypersensitive-like necroses of diverse plant species leading to resistance against fungal or bacterial plant pathogens. The solution structure of beta cryptogein from Phytophthora cryptogea fungus was determined by using multidimensional heteronuclear nuclear magnetic resonance spectroscopy. A set of 18 structures was calculated using 1360 NOE-derived distance restraints and 40 dihedral angle restraints obtained from 3JHNH alpha couplings. The RMS deviation from the mean structure is 0.87 +/- 0.14 A for backbone atoms and 1.34 +/- 0.14 A for all the non-hydrogen atoms of residues 2 to 98. The structure of beta cryptogein reveals a novel protein fold, with five helices and a double-stranded beta-sheet facing an omega-loop. One edge of the beta-sheet and the adjacent face of the omega-loop form a hydrophobic cavity. This cavity made of highly conserved residues represents a plausible binding site. Residue 13, which has been identified from directed mutagenesis and natural sequence comparison studies as a key amino acid involved in the differential control of necrosis, is surface exposed and could contribute to the binding to a ligand or a receptor. The solution structure is close to the X-ray structure, with slight differences lightly due to the crystal packing.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fefeu</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire de RMN, ICSN-CNRS, Gif-sur-Yvette, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouaziz</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Huet</LastName><ForeName>J C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Pernollet</LastName><ForeName>J C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Guittet</LastName><ForeName>E</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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MajorTopicYN="N">Solutions</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>2</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>2</Month><Day>12</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>2</Month><Day>12</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9385630</ArticleId><ArticleId IdType="pmc">PMC2143581</ArticleId><ArticleId IdType="doi">10.1002/pro.5560061101</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Phytochemistry. 1992 May;31(5):1471-6</Citation><ArticleIdList><ArticleId IdType="pubmed">1368359</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1991 Feb 5;217(3):517-30</Citation><ArticleIdList><ArticleId 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Bouaziz</name><name sortKey="Guittet, E" sort="Guittet, E" uniqKey="Guittet E" first="E" last="Guittet">E. Guittet</name><name sortKey="Huet, J C" sort="Huet, J C" uniqKey="Huet J" first="J C" last="Huet">J C Huet</name><name sortKey="Pernollet, J C" sort="Pernollet, J C" uniqKey="Pernollet J" first="J C" last="Pernollet">J C Pernollet</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Fefeu, S" sort="Fefeu, S" uniqKey="Fefeu S" first="S" last="Fefeu">S. Fefeu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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